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However, due to the high levels of autofluorescence in the cap1Δ/Δ background strain and the relatively low levels of Gpx3 production across all strains, we carried out shorter term assays in which cells were treated with t-BHP for 30 m which resulted in less autofluorescence in the cap1Δ/Δ strain than the 60 m treatment (Fig 4E)