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Microinjection and EV-treatment comparison Halo-Rac1 G12V protein was diluted to 300 μg/ml in a buffer containing 10 mM Tris-HCl, pH7.5, 150 mM NaCl, 5 mM MgCl 2 , and 6.25 mg/ml of 0.22 μm-filtered Rhodamine B isothiocyanate-Dextran (Sigma, R9379)